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My CartCAS：98-92-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 98-92-0 |
| Name | Nicotinamide |
| Molecular Formula | C6H6N2O |
| Molecular Weight | 122.12 |
| Solubility | Soluble in Water/DMSO ≥10mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 202-713-4 |
| MDL | MFCD00006395 |
| SMILES | O=C(C1=CC=CN=C1)N |
| InChIKey | DFPAKSUCGFBDDF-UHFFFAOYSA-N |
| InChI | InChI=1S/C6H6N2O/c7-6(9)5-2-1-3-8-4-5/h1-4H,(H2,7,9) |
| PubChem CID | 936 |
| Target Point | Sirtuin |
| Passage | Cell Cycle;DNA Damage/DNA Repair; Epigenetics |
| Background | Nicotinamide is a B3 vitamin that plays an important role in cellular physiology by promoting NAD+ redox homeostasis and providing NAD+ as a substrate for a class of enzymes that catalyze non-redox reactions. is an inhibitor of SIRT1. |
| Biological Activity | Nicotinamide是一种B3维生素，通过促进NAD +氧化还原稳态和提供NAD +作为催化非氧化还原反应的一类酶的底物，在细胞生理学中发挥重要作用。 烟酰胺是 SIRT1 的抑制剂。[1-4] |
| In Vitro | 用聚(ADP-核糖)聚合酶(PARP)抑制剂烟酰胺预处理能够预防HCN2细胞死亡。当在t-BuOOH之前加入烟酰胺时，它能够阻止神经元细胞死亡并抑制细胞凋亡。烟酰胺预处理的神经元具有更高的凋亡抑制因子(IAP)基因表达水平[1]。烟酰胺通过ET抑制血管收缩。烟酰胺还可以缓解氧化应激，从而加剧PE和FGR [3]。 |
| In Vivo | 正常和链脲佐菌素 - 烟酰胺诱导的成年男性糖尿病大鼠口服槲皮素(10，25和50 mg / kg / bw)，并导致FBG和心脏损伤标志物水平显着降低，胰岛素水平升高[2]。烟酰胺可改善RUPP小鼠的母体高血压，蛋白尿和肾小球内皮增生症。此外，烟酰胺可延长妊娠期，并可改善RUPP PE小鼠胚胎的存活和生长[3]。 |
| Animal Experiment | 在注射链脲佐菌素(STZ)(55mg / kg /体重)前15分钟，通过单次腹膜内(ip)注射溶解于生理盐水中的烟酰胺(110mg / kg /体重)诱导DM，将其溶解于新鲜制备的0.1mol / Lcitrate缓冲液(pH4.5)。这些注射是在禁食过夜后给出的。对照大鼠(n = 6)注射相同量的溶剂。为了在STZ注射后的最初24小时内防止低血糖，允许大鼠用5％葡萄糖(D5W)自由饮水。注射STZ-烟酰胺后3天，FBG水平大于7.0mM的大鼠被认为是糖尿病。 |
| Data Literature Source | [1]. Bhansali SG， et al. Nicotinamide prevents apoptosis in human cortical neuronal cells. Toxicol Mech Methods. 2006; 16 (4) :173-80. [2]. Roslan J， et al. Quercetin ameliorates oxidative stress， inflammation and apoptosis in the heart of streptozotocin-nicotinamide-induced adult male diabetic rats. Biomed Pharmacother. 2016 Dec 24; 86:570-582 [3]. Fushima T， et al. Nicotinamide ameliorates a preeclampsia-like condition in mice with reduced uterine perfusion pressure. Am J Physiol Renal Physiol. 2016 Dec 7:ajprenal.00501.2016 [4]. Suzuki E， et al. Protective effect of nicotinamide against poly (ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediated uptake. Life Sci. 2010 Apr 24; 86 (17-18) :676-82 |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in DMSO 10mM*1mL in Water 500mg |
Nicotinamide is a B3 vitamin that plays an important role in cellular physiology by promoting NAD + REDOX homeostasis and providing NAD + as a substrate for a class of enzymes that catalyze non-redox reactions. Nicotinamide is an indispensable nutrient in organoid culture. It is commonly used in the culture of gastrointestinal tract, liver and breast organoids to promote the formation of organoids and extend the life of organoids.
Examples of using this product（for reference only）
In Vitro:
Cell（human oral keratinocytes (HOKs) and human gingival epithelial cells (HGEs)；4-16 μg/ μL Nicotinamide/NAM；1h）:
The cells were cultured in DMEM, 100 U/ mL of penicillin, and 100 mg/mL of streptomycin supplemented with 20% FBS in 5% CO2 at 37℃ at a density of 5,000 cells per well in 96-well cell culture plates. The final concentrations of NAM were 4 μg/μL, 8 μg/μL, and 16 μg/μL and a medium without NAM served as negative control. After incubation under 5% CO2 at 37℃ for 1 h, the cells were washed with sterile PBS, and 0.1 mL of the CCK-8 reagent was added to each well. The plate was then incubated at 37℃ for 3 h. Then the absorbance was measured at 562 nm using a spectrometer. The statistics were normalized as cell via bility, and the result of the control group was set as 100%. Each concentration was tested in three wells, and the CCK-8 assays were performed in triplicate.
Reference：
Lin Y, Gong T, Ma Q, Jing M, Zheng T, Yan J, Chen J, Pan Y, Sun Q, Zhou X, Li Y. Nicotinamide could reduce growth and cariogenic virulence of Streptococcus mutans. J Oral Microbiol. 2022 Mar 23;14(1):2056291. doi: 10.1080/20002297.2022.2056291. PMID: 35341208; PMCID: PMC8956312.
In Vivo:
Rats（Sprague-Dawley rats；16 or 32 μg/μL Nicotinamide/NAM；2 min twice daily，4 weeks）:
The infected animals were randomly assigned into four groups of seven animals: (1) 32 μg/μL NAM, (2) 16 μg/μL NAM, (3) 250 μg/mL of fluoride (as positive control), and (4) ddH2O (as negative control). Animals in each group were treated with the compounds above topically using a camel hair brush for 2 min twice daily for 4 weeks. The rats were fed the cariogenic Keyes 2000 diet and 5% sucrose water ad libitum during the inoculation of S. mutans and the 4-week treatment. The rats’ weights were recorded to monitor for signs of toxicity.
Reference：
Lin Y, Gong T, Ma Q, Jing M, Zheng T, Yan J, Chen J, Pan Y, Sun Q, Zhou X, Li Y. Nicotinamide could reduce growth and cariogenic virulence of Streptococcus mutans. J Oral Microbiol. 2022 Mar 23;14(1):2056291. doi: 10.1080/20002297.2022.2056291. PMID: 35341208; PMCID: PMC8956312.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Lin Y, et al. J Oral Microbiol. 2022 Mar 23;14(1):2056291.
Lin Y, et al. J Oral Microbiol. 2022 Mar 23;14(1):2056291.
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