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My CartCAS：616-91-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 616-91-1 |
| Name | Acetylcysteine |
| Molecular Formula | C5H9NO3S |
| Molecular Weight | 163.19 |
| Solubility | Soluble in Water/DMSO ≥20mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 210-498-3 |
| MDL | MFCD00004880 |
| SMILES | SC[C@@H](C(O)=O)NC(C)=O |
| InChIKey | PWKSKIMOESPYIA-BYPYZUCNSA-N |
| InChI | InChI=1S/C5H9NO3S/c1-3(7)6-4(2-10)5(8)9/h4,10H,2H2,1H3,(H,6,7)(H,8,9)/t4-/m0/s1 |
| PubChem CID | 12035 |
| Target Point | Reactive oxygen species (ROS) |
| Passage | Immunology & Inflammation |
| Background | Acetylcysteine， an n-acetyl-derivative of cysteine， is a ROS inhibitor and mucolytic agent. Acetylcysteine induces apoptosis， can be used to reduce the thickness of mucus， and has anti-influenza virus activity. |
| Biological Activity | Acetylcysteine是一种粘液溶解剂，可以减少粘液的厚度。[1-5] |
| In Vitro | 在血清剥夺的PC12细胞中，N-乙酰半胱氨酸可以防止细胞凋亡的DNA片段化，并在没有其他营养支持的情况下维持长期存活。 N-乙酰半胱氨酸还可以预防PC12细胞和交感神经元的死亡[2]。 N-乙酰半胱氨酸导致大鼠和人主动脉平滑肌细胞活力的剂量依赖性降低[3]。 N-乙酰半胱氨酸激活PC12细胞中的Ras-细胞外信号调节激酶(ERK)途径。 N-乙酰半胱氨酸保护神经元细胞免于因营养支持的撤除而引起的死亡。 N-乙酰半胱氨酸增加血管组织中蛋白质结合储存的一氧化氮(NO)释放。 PC12细胞的N-乙酰半胱氨酸预处理干扰NGF依赖性信号传导和神经突向外生长，并且提示N-乙酰半胱氨酸干扰NGF机制中的氧化还原敏感步骤[4]。 |
| In Vivo | 在所有治疗组中，N-乙酰半胱氨酸(150，300 mg / kg)治疗显着降低肝转氨酶，主要是在N-乙酰半胱氨酸组300中。肺组织中谷胱甘肽过氧化物酶在N-乙酰半胱氨酸组300中显着增加(P = 0.04)，而其他氧化生物标志物没有显着差异[1]。 N-乙酰半胱氨酸可改善12个月大的SAMP8小鼠在T迷宫足部避免范例和杠杆按压食欲任务中的认知，而不会对运动活动，避免休克的动机或体重产生非特异性影响[5]。 |
| Cell Experiment | 对于存活实验，将洗涤的细胞重悬于RPM11640培养基中，并在涂有鼠尾胶原的24孔塑料培养皿中以每孔8-10×105的密度接种0.5mL。为了喂养，但为了避免漂浮细胞的损失，在第1，5和10天向培养物中加入新鲜培养基(0.2mL)。对于涉及“引发的”PC12细胞的实验，用NGF预处理培养物1-2周。在RPM1 1640培养基中补充1％热-iN-乙酰半胱氨酸化的马血清。然后洗涤细胞并传代到无血清的RPM11640培养基中。 |
| Animal Experiment | 大鼠：大鼠随机分为5组：假手术组(n = 5)，对照组IIR(n = 8)和3组IIR给予不同剂量的N-乙酰半胱氨酸：150 mg / kg腹腔注射5 min缺血(n = 8，N-乙酰半胱氨酸组150)，缺血前5分钟腹腔注射300 mg / kg(n = 7，N-乙酰半胱氨酸组300)，缺血前5分钟腹腔注射150 mg / kg加150 mg / kg 5再灌注前min(n = 7，N-乙酰半胱氨酸组150 + 150)。再灌注4小时后，通过腹主动脉放血使动物安乐死。 |
| Data Literature Source | [1]. Kalimeris K， et al. N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia reperfusion. J Surg Res. 2016 Dec; 206 (2) :263-272 [2]. Ferrari G， et al. N-acetylcysteine (D- and L-stereoisomers) prevents apoptotic death of neuronal cells. J Neurosci. 1995 Apr; 15 (4) :2857-66. [3]. Tsai JC， et al. Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. J Biol Chem. 1996 Feb 16; 271 (7) :3667-70. [4]. Yan CY， et al. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway. J Neurosci. 1998 Jun 1; 18 (11) :4042-9. [5]. Farr SA， et al. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice. J Neurochem. 2003 Mar; 84 (5) :1173-83 |
| Unit | Bottle |
| Specification | 250mg 10mM*1mL in DMSO 10mM*1mL in Water 500mg |
N-Acetyl-L-cysteine is a precursor of the antioxidant glutathione, which has antioxidant effects and is also a ROS inhibitor. It is required to be added during the culture of most organoids.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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您好，我用无菌水配制了NAC母液，细胞暴露时使用培养基稀释至所需浓度。发现2-10mM的NAC均能使本身为红色的培养基变成黄色，应该是NAC呈现酸性。请问此时需要调节其pH吗？又该如何调节呢？ 2023-05-24 09:26:46
Administrator您好，可以使用NaOH调节PH。