Modified Oil Red O Stain Kit
Cat.No：G1261 Solarbio
Storage：Store at 2-8℃，avoid light，6 months
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Product Information
| Name | Modified Oil Red O Stain Kit |
| Storage | Store at 2-8℃，avoid light，6 months |
| Unit | Kit |
| Specification | 2*50ml 2*100ml |
Product Introduction:
Oil Red O is a lipophilic azo dye that is a strong lipid solvent and dye, capable of specifically staining neutral triglycerides, lipids, and lipoproteins in tissues and cells. When tissue slices are inserted into the dye solution, the dye leaves the dye solution and dissolves in lipids (such as lipid droplets) within the tissue, causing the lipid droplets inside the tissue to turn red. Used to analyze the lipid status in cell samples.
The Modified Oil Red O Stain Kit is mainly used to display fat degeneration and abnormal deposition of lipid like substances in tissues and organs, which often occur in fatty degeneration of parenchymal organs such as liver, kidney, and heart, and most neutral fat droplets appear in cells; Identify and diagnose tumors and their properties that occur in adipose tissue. The specimen does not use a fixative containing ethanol (10% formalin can be used for fixation if necessary), nor does it use paraffin sections. Instead, frozen sections or carbon wax sections are required. The positive staining result of fat is orange yellow to red, and the specific color depends on the lipid concentration.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
脂肪肝造模冰冻切片
脂肪液滴着红色，细胞核着蓝色
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请问一下，脂肪染色除了中性脂肪（甘油三酯）外，其他脂质会被染色吗？比如胆固醇，游离脂肪酸等？因为我看到一些文章中提到，吞噬了游离脂肪酸的巨噬细胞也会被染色，这是为什么呢？谢谢 2022-07-21 20:39:31
Administrator1. 油红O一般与中性脂肪（也叫储存脂肪，主要是甘油三酯）有较强的结合能力，而与结构性脂肪（磷脂，糖脂及胆固醇）结合能力差。常规染色只显示胞内的中性脂肪。
2. 大量游离脂肪酸被细胞吞噬后经消化代谢，储存在胞内形成脂质沉积，呈现小液滴状分布在核周。而这些小液滴状的脂质含有大量甘油三酯，可与油红O亲和，所以显示橘红色。
可以用中性树胶封片吗 2022-07-14 14:17:12
Administrator不可以