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My Cart> Small Molecule Compounds > Mini Compound Kits > Stem Cell Correlation > Adipogenic Differentiation Small Molecules Kit-1（Powder,With Oil Red O and Indomethacin）
Storage：Store at -20℃
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| Name | Adipogenic Differentiation Small Molecules Kit-1（Powder,With Oil Red O and Indomethacin） |
| Storage | Store at -20℃ |
| Unit | Kit |
| Specification | 1Kit |
1. 本套餐精选了4种经典的可用于成脂诱导的基础试剂和高品质的油红O染料，均具有良好的生物活性。
2. 客户也可以根据自己的实验需求删减或添加其他所需的化合物。客户可根据自己的需求灵活定制专属Kit。
3. 已经过NMR、HPLC或MS测定，以确保化合物纯度和准确性。
基本操作（仅供参考）
1. 接种骨髓间充质干细胞：取对数生长期的细胞，按照2×104cells/cm2的细胞密度接种至包被后的培养器皿中，于37℃，5% CO2培养环境下培养至融合度90-100%，弃掉上清，加入成脂诱导分化培养基诱导液（含有地塞米松、胰岛素、吲哚美辛和IBMX）。
2. 细胞分化诱导：于37℃，5%CO2培养环境下培养约2-3天，更换为成脂诱导分化培养基维持液（只含有胰岛素）培养1天。
3. 按照以上换液频率继续诱导（一般是3个循环，约14天左右），并注意观察细胞形态变化。根据细胞诱导形成的脂滴数量和大小，决定终止细胞诱导的时间，并进行染色鉴定。
4. 细胞固定：吸去培养基,使用适量1×PBS清洗一次，弃去后取适量4%中性甲醛溶液覆盖培养器皿底面，室温固定30-60min，弃去固定液再使用1×PBS清洗两次。
5. 油红O染色：加入适量工作液，静置染色30min；吸走油红O染色液，用1×PBS清洗两次，并加入适量1×PBS避免细胞干燥。
6. 诱导评估：显微镜下观察成脂染色效果，并进行图像采集和诱导评估；诱导成功时，脂滴会与油红O染料结合后呈现红色或橘红色。
使用本产品的案例（仅供参考）
In Vitro
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
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