Mounting Medium, antifading (with Hoechst 33342)

Anti-fluorescence decay sealing agent is a kind of sealing reagent used to slow down fluorescence decay. Based on glycerol, the anti-fluorescence attenuator is added to have a strong anti-fluorescence attenuator effect, which is used for sealing fluorescent tissue and cell samples. After sealing, the sample can be stored at 4℃ or -20℃ away from light for 2-3 weeks. The reagent is simple to operate, and it can be used to drop a drop of anti-fluorescence attenuation sealing solution with a pipette gun and cover the cover glass.

The anti-fluorescence attenuation tablet contains Hoechst 33342, also known as bisBenzimide H 33342 or HOE 33342, which is a blue fluorescent dye that penetrates cell membranes and is less toxic to cells. Hoechst 33342 staining is often used to detect apoptosis, which is observed by fluorescence microscopy or flow cytometry after staining. Hoechst 33342 is also commonly used for conventional nuclear staining, or conventional DNA staining. Hoechst 33342 has a maximum excitation wavelength of 346nm and a maximum emission wavelength of 460nm. Hoechst 33342 combined with double-stranded DNA has a maximum excitation wavelength of 350nm and a maximum emission wavelength of 461nm.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

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3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.