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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

BC5510Manual

Cat.No：BC5510 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
Detection Method	Spectrophotometer
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/48S

Determination of Significance：

Mitochondrial aldehyde dehydrogenase 2 (ALDH2) belongs to the aldehyde dehydrogenase protein family, which exists in many tissues, especially the liver. This enzyme is mainly involved in the second step of ethanol metabolism process, which oxidizes acetaldehyde into carboxylic acid in mitochondria, and then enters the tricarboxylic acid cycle, which is completely decomposed to remove the toxic effect of acetaldehyde on organisms. In addition, ALDH2 can also participate in the metabolism of nitroglycerin as an esterase, and is an important bioactive agent of nitroglycerin.

Measurement Principle：

ALDH2 catalyzes the conversion of acetaldehyde and NAD+ to acetic acid and NADH, and the activity of ALDH2 can be calculated by using the change of absorption value of NADH at 340nm.

Self Provided：

Ultraviolet spectrophotometer, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, 1 mL quartz cuvette, mortar /homogenizer/ sonicator, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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