Krebs-Ringer Buffer
Cat.No：G0430 Solarbio
Appearance：Colourless transparent liquid
Storage：Store at 2-8℃，avoid light，1 year.
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HIF
Immunology & Inflammation
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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Product Information
| Name | Krebs-Ringer Buffer |
| Storage | Store at 2-8℃，avoid light，1 year. |
| Appearance | Colourless transparent liquid |
| Unit | Bottle |
| Specification | 500ml |
This product contains glucose and does not contain calcium chloride, please refer to the instructions for more details.
Product type: Sterile filter ready-to-use liquid medium
Osmotic pressure: 270 ± 15 Acid alkalinity: 7.3 ± 0.2
Description:
As early as the 19th century, researchers explored how to grow animal tissues in vitro, and balanced salt solution was an early solution used in tissue culture. In 1985, Sydney Ringer prepared a salt solution consisting entirely of inorganic salt components to successfully maintain contractility of mammalian myocardial tissue in vitro. Tyrode then prepared a low specificity Tyrode salt solution that was widely used in mammalian cell work, and this medium was widely accepted at the time as a mother solution for diluting other components, such as proteins. Since then, a variety of balanced salt solutions have been developed for tissue and cell culture, and according to their role, the currently commonly used balanced salt solutions are divided into the following categories:1) Provide water and inorganic salts for cell growth and proliferation to meet normal cell metabolism;
2) to provide a suitable acid-base environment for cell growth, the general pH range is between 7.2 and 7.6;
3) Provide amino acids and other nutrients to meet the needs of cell growth, proliferation and metabolism;
4) Add glucose and metabolic intermediates to meet the energy needs of cells;
5) to provide a suitable osmotic pressure environment for cells.
References:
Krebs HA and Henseleit K. Studies on urea formation in the animal organism. Hoppe-Seylers Z.Physiol. Chem. 210:33-66, 1932.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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