Senescence-Associated β-Galactosidase（SA-β-Gal） Stain Kit
Cat.No：G1580 Solarbio
Storage：Store at -20℃，avoid light，1 year
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> Dye Reagents > Enzymatic Stain > Senescence-Associated β-Galactosidase（SA-β-Gal） Stain Kit
Product Information
| Name | Senescence-Associated β-Galactosidase（SA-β-Gal） Stain Kit |
| Storage | Store at -20℃，avoid light，1 year |
| Delivery Time | 2-3 days |
| Unit | Kit |
| Specification | 100T |
Product Introduction:
The Senescence β-Galactosidase Staining Kit is a staining kit for senile cells or tissues based on the up-regulation of SA-β-Gal(senescence-associated β-galactosidase) activity during aging. Aging of cells or tissues can be observed under optical microscopes. Cell aging is also considered as a way for organisms to inhibit tumors and a potential cause of organism aging. The kit can be used for senescence detection of cultured cells and tissue sections.
Using X-Gal as substrate, the Senescence β-Galactosidase Staining Kit produces dark blue products catalyzed by aging-specific β-galactosidase. Cells or tissues expressing β-galactosidase that turn blue are easily observed under optical microscopy. This kit only stains senescent cells. For tissue sections or blocks, the number of samples that can be detected depends on the size of the sample. For ordinary sections or 6-well plate, at least 100 samples can be detected.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
损伤造模悬浮细胞涂片酶活性位点着绿色，红色复染液复染后呈蓝色。细胞呈淡黄色或细胞本色
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我染肿瘤细胞，看这个说明书，肿瘤细胞是不是不太好染 2023-05-26 11:41:45
Administrator您好，是的，肿瘤细胞，永生细胞等细胞不太好染色。
SA-β-gal染色可以染石蜡切片还是冰冻切片 2022-08-02 15:06:16
Administrator您好，建议做冰冻切片，对酶活性保护效果更好，石蜡切片来做必须要使用低熔点石蜡，在制片过程中对酶的活性影响相对更大一些。