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Granule-Bound Starch Synthase（GBSS）Activity Assay Kit

BC3295Manual

Cat.No：BC3295 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Granule-Bound Starch Synthase（GBSS）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Granule-Bound Starch Synthase (GBSS，EC 2.4.1.21) is present in the amyloid body in a bound state, catalyzing the elongation reaction of the starch chain, and is mainly responsible for the synthesis of amylose.

Measurement Principle：

GBSS catalyzes the reaction of ADPG with starch primer (glucan), transferring glucose molecules to starch primers, and simultaneously generating ADP. Further, the pyruvate kinase, hexokinase and glucose-6-phosphate dehydrogenase added in the reaction system sequentially catalyze the reduction of NADP+ to NADPH, wherein the amount of NADPH is proportional to the amount of ADP produced by the previous reaction, and the NADPH is measured at 340 nm. Increase the amount to calculate GBSS activity.

Self Provided：

Spectrophotometer/Microplate Reader, Water Bath, Centrifuge, Transferpettor, Micro Quartz Cuvette/96 Well Flat-Bottom Plate (UV plate), Mortar, Ice and Distilled Water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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