Soil Acid Invertase(S-AI) Activity Assay Kit
Cat.No：BC3075 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Soil Series > Soil Acid Invertase(S-AI) Activity Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Soil Acid Invertase(S-AI) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
S-AI catalyzes the irreversible decomposition of sucrose into fructose and glucose at pH 4.5 to 5.0 (acidic). It is one of the key enzymes for sucrose metabolism in soil microorganisms.
Measurement Principle：
S-AI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to NI activity. The activity of S-NI is calculated by the increasing rate of light absorption.
Self Provided：
Spectrophotometer/ Microplate reader, centrifuge,constant temperature incubator/water-bath, transferpettor, micro glass cuvette/ 96 well flat-bottom plate, mortar/ homogenizer, toluene, sieve (50 mesh, or smaller) ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Effects of mushroom-tobacco rotation on microbial community structure in continuous cropping tobacco soil
Click to check >>Author：Wang Xianju, Li Zhihua, Jin Honggang, Huang Xiaoming, Xiao Xubin, Zi Shuhui, Liu Tao
IF：4.0590
Publish_to：JOURNAL OF APPLIED MICROBIOLOGY
PMID：37156515
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