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D-lactate Dehydrogenase（D-LDH）Activity Assay Kit

BC5285Manual

Cat.No：BC5285 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	D-lactate Dehydrogenase（D-LDH）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Lactate dehydrogenase (LDH) is the terminal enzyme of the glycolysis pathway which is widely found in animals, plants, microorganisms and cultured cells. LDH catalyzes the reversible conversion of lactate to pyruvic acid with the reduction of NAD+ to NADH and vice versa. According to the different configuration of catalytic substrate, it could be divided into D-lactate dehydrogenase (D-LDH, EC1.1.1.28) and L-lactate dehydrogenase (L-LDH, EC1.1.1.27).

Measurement Principle：

NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of D-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.

Self Provided：

Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer/cell ultrasonic crusher, ice, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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