Cytochrome b5 Content Assay Kit
Cat.No：BC2765 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Energy Metabolism Series > P450 Series > Cytochrome b5 Content Assay Kit
Product Information
| Name | Cytochrome b5 Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Cytochrome P450 enzymes are a group of isozymes mainly found in the liver and play an important role in the metabolism of exogenous substances, especially drugs and poisons. Cytochrome P450 and cytochrome b5 are two heme proteins in the P450 enzyme system, and the change of their ratios is closely related to the metabolic activity of P450.
Measurement Principle：
After the oxidized cytochrome B5 is reduced by sodium dithionite, it has a maximum absorption peak at 424 nm, and the content of cytochrome B5 can be calculated by measuring the difference in absorbance values between 424 nm and 490 nm.
Self Provided：
Visible spectrophotometer/microplate reader, cryogenic centrifuge, ultracentrifuge, thermostatic incubator, mortar/homogenizer, adjustable pipette, microglass cuvette/96-well plate, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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