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Pectin Lyase（PL）Activity Assay Kit

BC2645Manual

Cat.No：BC2645 Solarbio

Storage：Store at 2-8℃，6 months

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Product Information

Name	Pectin Lyase（PL）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Pectin lyase (EC4.2.2.10) is an important part of pectinase and catalyzes the elimination and cleavage of pectin molecular chain. The sources are relatively wide, mainly derived from microorganisms, which is of great significance in increasing fruit juice production in the food processing industry, and also has potential application value in reducing environmental pollution and energy consumption.

Measurement Principle：

Pectin lyase acts on the α-1,4 glycosidic bond in pectin, and generates unsaturated oligogalacturonic acid having an unsaturated bond between the reducing end C4 and C5. It has a characteristic absorption peak at 235nm. The increase in absorbance at 235 nm is measured to indicate the activity of pectin lyase.

Self Provided：

Spectrophotometer/microplate reader, centrifuge, constant temperature water bath, transferpettor, micro quartz cuvette/96 well UV plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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