Sorbitol Dehydrogenase(SDH) Activity Assay Kit
Cat.No：BC2535 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > Sorbitol Dehydrogenase(SDH) Activity Assay Kit
Product Information
| Name | Sorbitol Dehydrogenase(SDH) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
SDH (EC 1.1.1.14) catalyzes the dehydrogenation of sorbitol to fructose, which is one of the key enzymes to regulate the content of sorbitol in vivo.
Measurement Principle：
SDH catalyzes the dehydrogenation of sorbitol to fructose, and the reduction of NAD+ to NADH. The generated NADH can transfer electrons to NBT to generate purple hairpin. According to this principle, SDH activity can be calculated.
Self Provided：
Spectrophotometer/Microplate reader, desk centrifuge, water bath/ constant temperature incubator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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