Phospholipase D（PLD）Activity Assay Kit
Cat.No：BC2415 Solarbio
Storage：Store at -20℃，6 months
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Phospholipase D（PLD）Activity Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Phospholipase D（PLD）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Phospholipase D catalyzes the hydrolysis of phosphatidyl diester bond at the end of phosphatidylcholine to form phosphatidylic acid and choline. Choline is catalyzed by choline oxidase to form betaine and hydrogen peroxide. Hydrogen peroxide oxidizes 4-aminoantipyrine and bisphenol to pink substance under the action of catalase. The pink substance is a characteristic absorption peak at 500 nm.
Measurement Principle：
Phospholipase D catalyzes the hydrolysis of phosphatidyl diester bond at the end of phosphatidylcholine to form phosphatidylic acid and choline. Choline is catalyzed by choline oxidase to form betaine and hydrogen peroxide. Hydrogen peroxide oxidizes 4-aminoantipyrine and bisphenol to pink substance under the action of catalase. The pink substance is a characteristic absorption peak at 500 nm.
Self Provided：
Spectrophotometer/microplate reader, Balance, Mortar/Cell ultrasonic crusher, Ultra freezing centrifuge, Micro Quartz Cuvette/96 Well UV Flat-bottom Plate, Constant temperature water bath, Anhydrous ethanol, Ice.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Transcriptomics and metabolomics provide insight into the anti-browning mechanism of selenium in freshly cut apples
Click to check >>Author：Xinyue Wang, Xuemei Zhang, Peng Jia, Haoan Luan, Guohui Qi, Han Li, Suping Guo
IF：6.6270
Publish_to：Frontiers in Plant Science
PMID：37223812
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