Fructose-bisphosphate aldolase(FBA) Activity Assay Kit
Cat.No：BC2275 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Fructose-bisphosphate aldolase(FBA) Activity Assay Kit
Product Information
| Name | Fructose-bisphosphate aldolase(FBA) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Fructose 1,6 bisphosphate aldolase (FBA) (EC4.1.2.13) is an important part of the Calvin cycle in glycolysis, gluconeogenesis, pentose phosphate pathway, and photosynthesis Enzymes, which catalyze the reversible cleavage of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, are widely present in plants, animals and microorganisms, and exhibit different responses under various stress conditions.
Measurement Principle：
FBA catalyzes the production of fructose 1,6-diphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate, and catalyzes NADH and triphosphate under the action of triose phosphate isomerase and α-phosphate glycerol dehydrogenase. Dihydroxyacetone phosphate produces NAD and α-phosphoglycerin. The change in absorbance at 340nm can reflect the level of fructose 1,6-bisphosphate aldolase activity.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, adjustable transferpettor, micro quartz cuvette/ 96 well UV plate, mortar/homogenizer, vortex oscillator, ultrasonic crusher, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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