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Glyceraldehyde-3-phosphate Dehydrogenase(GAPDH) Activity Assay Kit

BC2215Manual

Cat.No：BC2215 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Glyceraldehyde-3-phosphate Dehydrogenase(GAPDH) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

GAPDH (EC 1.2.1.12) catalyzes the oxidation of glyceraldehyde 3-phosphate to 1,3-diphosphoglyceride. It is the key enzyme of glycolysis pathway. It is closely related to the pathway of gluconeogenesis, the maintenance of blood glucose concentration and the occurrence of diabetes. It plays an important role in the disorders of glucose, lipid and protein metabolism.

Measurement Principle：

3-phosphoglycerate kinase can catalyze the production of 1,3-diphosphoglyceride from triphosphate and ATP. GAPDH reversely catalyzes the formation of glyceraldehyde-3-phosphate, inorganic phosphorus and NAD+ from 1,3-diphosphoglyceride and NADH. The decrease of NADH measured at 340 nm can reflect the activity of GAPDH.

Self Provided：

Desk centrifuge, ultraviolet spectrophotometer/microplate reader, constant temperature water bath, mortar/ homogenizer, micro quartz cuvette/96 well UV plate, transferpettor, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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