Phosphoenolpyruvate Carboxylase(PEPC) Activity Assay Kit
Cat.No：BC2195 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Phosphoenolpyruvate Carboxylase(PEPC) Activity Assay Kit
Product Information
| Name | Phosphoenolpyruvate Carboxylase(PEPC) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Phosphoenolpyruvate carboxylase (EC 4.1.1.31) is widely found in plants and microorganisms. It catalyzes the irreversible reaction of phosphoenolpyruvate and carbon dioxide to oxaloacetic acid. It is also a key enzyme for C4 plants and cam plants to fix CO2. It plays an important role in regulating the operation of tricarboxylic acid cycle.
Measurement Principle：
PEPC can catalyze phosphoenolpyruvate and carbon dioxide to form oxaloacetate and HPO42-. Malate dehydrogenase can catalyze oxaloacetate and NADH to produce malate and NAD+. The rate of NADH reduction was measured at 340 nm and PEPC activity was calculated.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, pipette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Exogenously applied zinc improves sugar-acid profile of loquat (Eriobotrya japonica Lindl.) by regulating enzymatic activities and expression of their metabolism-related genes
Click to check >>Author：Muhammad Moaaz Ali, Shaista Gull, Xiaobo Hu, Youming Hou, Faxing Chen
IF：6.5000
Publish_to：PLANT PHYSIOLOGY AND BIOCHEMISTRY
PMID：37329690
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