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Isocitrate Dehydrogenase Mitochondrial(ICDHm) Activity Assay Kit

BC2165Manual

Cat.No：BC2165 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Isocitrate Dehydrogenase Mitochondrial(ICDHm) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Isocitrate dehydrogenase (ICDHm) is widely present in mitochondria of animals, plants, microorganisms and cultured cells, and is related to mitochondrial gene expression and other functions of mitochondria. There are two forms of isocitrate dehydrogenase in the body, NAD-dependent isocitrate dehydrogenase using NAD as a coenzyme, and NADP-dependent isocitrate dehydrogenase using NADP as a coenzyme.

Measurement Principle：

The main function of isocitrate dehydrogenase is which catalyze the production of α-ketoglutarate from isocitrate during the tricarboxylic acid cycle in the body, reduce NAD to NADH. Isocitrate dehydrogenase activity could be calculate by determine the amount of α-ketoglutarate produced.

Self Provided：

Centrifuge, spectrophotometer/plate reader, water bath/incubator, micro glass cuvettes/96-well plate, adjustable pipette, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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