Total Cholesterol(TC) Content Assay Kit
Cat.No:BC1985 Solarbio
Storage:Store at 2-8℃,6 months
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Fatty Acid Metabolism Series >
Total Cholesterol(TC) Content Assay KitStorage:Store at 2-8℃,6 months
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Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
Name | Total Cholesterol(TC) Content Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Total cholesterol (TC) is the sum of cholesterol contained in all lipoproteins, including free cholesterol and cholesteryl esters.
Measurement Principle:
The enzyme esterase catalyzes the hydrolysis of cholesteryl esters to produce free cholesterol (FC) and free fatty acids (FFA), thus converting cholesteryl esters to FC; furthermore, cholesteryl oxidase catalyzes the oxidation of FC to produce 4-Cholestenone and H2O2; finally, peroxidase catalyzes the oxidation of 4-aminoantipyrine and phenol by H2O2 to produce red quinones, which have a characteristic absorption peak at 500 nm. The color shade is proportional to the TC content.
Self Provided:
Spectrophotometer/microplate reader, balance, low temperature table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, pipette, EP tube, distilled water, isopropyl alcohol.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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2023-08-10 11:44:41
请问用组织提取方法提取肝脏中的总胆固醇后,再用试剂盒进行检测时出现大量白色混浊物影响吸光度如何处理呢 2023-05-25 08:42:57
Administrator您好,需要使用本试剂盒中提取液,按照说明书中组织样本处理方式处理样本。