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Micro Soluble Starch Synthase(SSS)Assay Kit

BC1855Manual

Cat.No：BC1855 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Micro Soluble Starch Synthase(SSS)Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Soluble Starch Synthase (SSS, EC 2.4.1.21) usually present in the free matrix in the plastid matrix, which catalyzes the elongation of the starch chain, mainly responsible for the synthesis of amylopectin.

Measurement Principle：

SSS catalyzes the reaction of ADPG with starch primer (glucan), transfers glucose molecules to starch primers, and simultaneously produces ADP. Add pyruvate kinase, hexokinase and 6-phosphate glucose dehydrogenase to the reaction system. These enzymes in turn catalyze NADP+ reduction to NADPH, the amount of NADPH produced is proportional to the amount of ADP produced in the previous step reaction, and the SSS activity can be calculated by measuring the increase of NADPH at 340 nm.

Self Provided：

Spectrophotometer/Microplate Reader, Water Bath, Desk Centrifuge, Transferpettor, Micro Quartz Cuvette/96 Well UV Plate, Mortar/Homogenizer, Ice and Distilled Water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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