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Application：ELISA
Reactivity：Rabbit
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15pg/mL
Storage：2-8℃，6 months
Gene ID：100009087
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| Name | Rabbit ANP ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | ANP |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rabbit |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.2-2，000pg/mL |
| Sensitivity | 15pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h30min |
| Swiss Prot | P07500 |
| Gene ID | 100009087 |
| Background | Atrial natriuretic peptide (ANP) is a powerful vasodilator，and a protein hormone secreted by heartmuscle cells. It is involved in the homeostatic control of body water，sodium，potassium and adiposetissue. It is released by atrial myocytes in response to high blood volume. ANP acts to reduce the water，sodium and adipose loads on the circulatory system，thereby reducing blood pressure. ANP has exactlythe opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodiumin the kidney that is，aldosterone stimulates sodium retention and ANP generates sodium loss.Incardiac myocytes，ANP is made as a precursor form，i.e. prepro-ANP，a polypeptide of 151 aminoacids. After the signal peptide is removed in the endoplasmic reticulum，the 126-amino-acid pro-ANPis stored in the intracellular granules. When the cells are stimulated，pro-ANP is released and converted tothe 28-amino-acid C-terminal mature ANP on the cell surface by the cardiac transmembrane serineprotease corin.ANP interacts with three receptors including guanylyl cyclase-A (NPR1)，guanylylcyclase-B (NPR2)，natriuretic peptide clearance receptor (NPR3). The binding of ANP to its receptorcauses the conversion of GTP to cGMP and raises intracellular cGMP. Receptor-agonist binding causes areduction in blood volume and，therefore，a reduction in cardiac output and systemic blood pressure.Lipolysis is increased and renal sodium reabsorption is decreased. The overall effect of ANP on the bodyis to counter increases in blood pressure and volume caused by the renin-angiotensin system. ANP andrelated peptides are used as biomarkers for cardiovascular diseases such as stroke，coronary arterydisease，myocardial infarction and heart failure. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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