Magrose Nhs Kit

Magrose NHS is surface modified with NHS groups and can form stable peptide bonds with proteins and other molecules with primary amine groups for affinity purification of antibodies, antigens and other biomolecules. Compared with traditional carboxyl and amino magnetic beads, the beads containing NHS groups do not need to be activated by EDC/NHS or glutaraldehyde in advance. The primary amino containing biological ligand can simply be dissolved in the Coupling Buffer provided with the kit. By mixing the protein with NHS magnetic beads at room temperature for 1~2 h, the biological ligand can be covalently coupled to the magnetic beads, which has the advantages of simple operation, mild coupling conditions, fast and efficient biological ligand coupling. The magnetic bead coupling process must be carried out in a buffer solvent without any amino group. In manual operation, the magnetic separation frame is used to separate the magnetic bead from the solvent. It can also be operated with automated equipment, which is suitable for the screening of multiple samples.

Product advantages

1. Simple, no activation required, can be directly covalently coupled with biological ligand;

2. High efficiency, the coupling efficiency of biological ligand can reach more than 90%, which is much higher than that of carboxyl magnetic beads, and the binding load of ligand is higher;

3. Fast, 1~2h can complete the biological ligand coupling;

4. Mild, room temperature or 4℃ coupling, coupling system pH 5~9;

5. Stable, forming a stable amide bond to prevent the ligand from falling off.

6. Good biocompatibility, reduce non-specific adsorption.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.