Diamine Oxidase(DAO) Activity Assay Kit
Cat.No：BC1285 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Oxidation Series > Diamine Oxidase(DAO) Activity Assay Kit
Product Information
| Name | Diamine Oxidase(DAO) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
DAO (EC1.4.3.6) is widely found in animals (intestinal mucosa, lung, liver, kidney, etc.), plants, and microorganisms. Catalytic oxidation of polyamines to aldehydes, whose activity is closely related to nucleic acid and protein synthesis, can reflect the integrity and damage degree of the intestinal mechanical barrier.
Measurement Principle：
DAO catalyzed cadaverine to produce aldehydes and hydrogen peroxide, and Fe2+ in potassium ferricyanide oxidized by H2O2 to produce Fe3+. Fe3+ reacted with 4-amino-antipyrine and phenol to produce red quinones with characteristic absorption peaks at 505nm. The activity of DAO was calculated by measuring the absorbance value at 505nm.
Self Provided：
Spectrophotometer/Microplate reader, mortar/homogenizer/cell ultrasonic crusher, centrifuge, constant temperature foster box/water-bath, centrifuge, micro quartz cuvette/96 well flat-bottom plate, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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