Ascorbic Acid Oxidase(AAO) Activity Assay Kit
Cat.No：BC1265 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Vitamin C Metabolism Series > Ascorbic Acid Oxidase(AAO) Activity Assay Kit
Product Information
| Name | Ascorbic Acid Oxidase(AAO) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
AAO is a glycoprotein that located in plant cell wall, belong to the "blue copper oxidase" family. Ascorbic acid and AAO in the cell wall are closely related to cell wall metabolism and growth. AAO catalyzes the oxidation of AsA to MDHA, which can be reduced by cytochrome b on the plasma membrane. The transmembrane transport of electrons in this process can promote cell growth.
Measurement Principle：
AAO can directly oxidize AsA. The activity of AAO can be calculated by measuring the oxidation amount of AsA.
Self Provided：
Low temperature centrifuge, ultraviolet spectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom plate (UV plate), adjustable pipette, mortar, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Involvement of Nitric Oxide and Melatonin Enhances Cadmium Resistance of Tomato Seedlings through Regulation of the Ascorbate–Glutathione Cycle and ROS Metabolism
Click to check >>Author：Junrong Xu, Zhien Wei, Xuefang Lu, Yunzhi Liu, Wenjin Yu, Changxia Li
IF：6.2080
Publish_to：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：37298477
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