Glutathione Reductases(GR) Activity Assay Kit
Cat.No：BC1165 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Glutathione Series > Glutathione Reductases(GR) Activity Assay Kit
Product Information
| Name | Glutathione Reductases(GR) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
GR is a flavor-protein oxidoreductase widely existing in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH, which is one of the key enzymes of glutathione redox cycle (GR is usually replaced by TrxR in insects). GR catalyzes the reduction of GSSG to generate GSH by NADPH, which is helpful to maintain the body's GSH/GSSG ratio.GR plays a key role in the scavenging of reactive oxygen species in oxidative stress. In addition, GR also participates in thecycle pathway of ascorbic acid and glutathione.
Measurement Principle：
GR catalyzes the reduction of GSSG by NADPH to produce GSH, at the same time, NADPH dehydrogenation produces NADP+. NADPH has a characteristic absorption at 340 nm. On the contrary, NADP+ has no absorption peak at this wavelength. The rate of NADPH dehydrogenation is determined by measuring the rate of decrease of absorbance at 340 nm, thereby calculating GR activity.
Self Provided：
Spectrophotometer/Microplate reader, low temperature centrifuge, water bath, adjustablepipette, micro quartz cuvette/96 well flat-bottomplate (UV plate) and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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