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My Cart> Antibodies > Loading Control Antibodies > Nucleus > Anti-HIST1H3A Polyclonal AntibodyReactivity：Mouse,Rat,Monkey,Human
Application： IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-HIST1H3A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:8000-12000.;IHC 1:50-200. |
| Target | HIST1H3A |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Swiss Prot | P68431 |
| Gene ID | 8350 |
| Subcellular Location | Nucleus Secreted |
| Immunogen | Recombinant protein of human HIST1H3A |
| Public Immunogen Range | 1-137aa |
| Calculated MW | 15kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Y-Dilution Ratio | WB 1:8000-12000. IHC 1:50-200. |
| Specification | 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HIST1H3A antibody diluted at 1:10000.Lane: HEK293,A549,293T,HeLa,COS7,NIH/3T3,Raji,HepG2,Rat lung,Mouse spleen.
Immunohistochemistry of paraffin-embedded Human breast cancer with HIST1H3A antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human prostate cancer with HIST1H3A antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human renal cancer with HIST1H3A antibody diluted at 1:100.
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