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NAD Malic Enzyme(NAD-ME) Activity Assay Kit

BC1135Manual

Cat.No：BC1135 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	NAD Malic Enzyme(NAD-ME) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

MEwidely exists in microbe, culturing cells, animal and plantcytoplasm. Especially it has high activity in plant tissue. ME catalyzesthe reversible reaction of malic acidoxidative decarboxylation to produce pyruvic acid and CO2with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity and substrate specificity, ME is divided into NAD-ME(EC1.1.1.38) and NADP-ME(EC1.1.1.40).

Measurement Principle：

NADP-ME catalyzesthe reduction of NADP+ to NADPH,detect the increase rate of NADPH at 340nm.

Self Provided：

Spectrophotometer/microplate reader, low temperature centrifuge, water bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate(UV plate), mortar/homogenizer and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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