NADP Malic Enzyme(NADP-ME) Activity Assay Kit
Cat.No：BC1125 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme Ⅱ Series > NADP Malic Enzyme(NADP-ME) Activity Assay Kit
Product Information
| Name | NADP Malic Enzyme(NADP-ME) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
MEwidely exist in microbe, culturing cells, animal and plant cytoplasm. Especially it has high activity in plant tissue. ME catalyzes the reversible reaction of malic acid oxidative decarboxylation to produce pyruvic acid and CO2 with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity and substrate specificity, ME is divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40).
Measurement Principle：
NADP-ME catalyzes the reduction of NADP+ to NADPH, detect the increase rate of NADPH at 340 nm.
Self Provided：
Spectrophotometer/Microplate reader, low temperature centrifuge, water-bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate(UV plate), mortar/homogenizer and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
HMGA2 knockdown alleviates the progression of nonalcoholic fatty liver disease (NAFLD) by downregulating SNAI2 expression
Click to check >>Author：Muhammad Moaaz Ali, Shaista Gull, Xiaobo Hu, Youming Hou, Faxing Chen
IF：6.5000
Publish_to：PLANT PHYSIOLOGY AND BIOCHEMISTRY
PMID：37329690
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