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Alcohol Dehydrogenase(ADH) Activity Assay Kit

BC1085Manual

Cat.No：BC1085 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Alcohol Dehydrogenase(ADH) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Alcohol dehydrogenase (ADH) is a key enzyme in the metabolism of short chain alcohols. It catalyzes the reversible conversion of ethanol and acetaldehyde, and plays an important role in many physiological processes. In mammals, ADH is mainly produced in the liver. Liver damage causes ADH to be released into serum. The activity of serum ADH reflects whether the liver function is abnormal.

Measurement Principle：

ADH catalyzes the reduction of acetaldehyde by NADH to ethanol and NAD+. NADH has an absorption peak at 340 nm but NAD+ not, the activity of ADH is calculated by measuring the rate of absorbance decline at 340 nm.

Self Provided：

Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice, Low Temperature Centrifuge, Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/96 Well Flat-bottom Plate (UV plate), Water Bath; Adjustable Pipette and Distilled Water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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