Pyruvate Decarboxylase(PDC) Activity Assay Kit
Cat.No：BC1075 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Pyruvate Decarboxylase(PDC) Activity Assay Kit
Product Information
| Name | Pyruvate Decarboxylase(PDC) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol fermentation.
Measurement Principle：
PDC catalyzes pyruvate decarboxylation to form acetaldehyde. The addition of alcohol dehydrogenase (ADH) can further catalyze the reduction of aldehydes by NADH to ethanol and NAD+. NADH has an absorbance at 340 nm but NAD+ not, the activity of PDC can be calculated by measuring decrease rate of absorption at 340 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/ 96 Well Flat-bottom Plate (UV Plate), Water-bath/Constant Temperature Incubator, Desk Centrifuge, Adjustable Pipette, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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