Fructose 1,6-bisphosphatase(FBP) Activity Assay Kit
Cat.No：BC0925 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Gluconeogenic Series > Fructose 1,6-bisphosphatase(FBP) Activity Assay Kit
Product Information
| Name | Fructose 1,6-bisphosphatase(FBP) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Fructose 1,6-bisphosphatase(FBP) also known as fructose-1,6-diphosphatase, which plays a key role in the gluconeogenesis and the synthesis of photosynthetic assimilate sucrose.
Measurement Principle：
FBP catalyzes fructose 1,6-diphosphate and water to produce 6-phosphate fructose and inorganic phosphorus. Glucose-phosphate isomerase and 6-glucose-phosphate dehydrogenase added to the reaction system that catalyze the formation of 6-glucose-phosphate gluconic acid and NADPH in turn. In this kit, the activity of FBP is determined by the increase rate of NADPH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/Microplate Reader, desk centrifuge, adjustable pipette, water bath, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
A novel variant in the FBP1 gene causes fructose-1,6-bisphosphatase deficiency through increased ubiquitination
Click to check >>Author：Xiaoyan Liang, Xiaoliang Liu, Wenjing Li, Lu Zhang, Bijun Zhang, Guangrui Lai, Yanyan Zhao
IF：4.1140
Publish_to：ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
PMID：37142076
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