Glutamine Synthetase(GS) Activity Assay Kit
Cat.No：BC0915 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Nitrogen Metabolism Series > Other Nitrogen Metabolism Series > Glutamine Synthetase(GS) Activity Assay Kit
Product Information
| Name | Glutamine Synthetase(GS) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Glutamine synthetase (GS) mainly exists in plants, is one of the key enzymes of ammonia assimilation in organism, which can catalytic synthesis of glutamine by ammonium ion and glutamic acid. The synthesis of glutamine not only prevents excessive ammonium ions from being toxic to organisms, but glutamine is also the main storage and transport form of ammonia.
Measurement Principle：
GS catalyzes the synthesis of glutamine from ammonium and glutamic acid in the presence of ATP and Mg2+. Glutamine is further converted to gamma-glutamyl hydroxamic acid, which can form a red complex with iron under acidic condition. This complex has a maximum absorption peak at 540 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Water Bath, Desk Centrifuge, Adjustable Pipette, Micro Glass Cuvette/96 Well Flat-bottom Plate, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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