Carboxylesterase(CarE) Activity Assay Kit
Cat.No:BC0845 Solarbio
Storage:Store at -20℃,6 months
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Carboxylesterase(CarE) Activity Assay KitStorage:Store at -20℃,6 months
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Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
Name | Carboxylesterase(CarE) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Mammalian CarE also known as aliphatic esterase (aliesterase) is widely distributed in tissue and organism, belonging to the serine hydrolase family. CarE catalyze hydrolysis of endogenous and exogenous substances containing ester bonds, amide bonds, and thioester bonds, but can’t catalyze hydrolysis of acetylcholine and its analogues. CarE take part in lipid transport and metabolism, and related with detoxification and metabolism of many drugs, environmental poisons and carcinogens. Organophosphorus pesticides can bind to CarE and inhibit CarE activity.
Measurement Principle:
CarE can catalyze acetic acid-1-naphthalene ester to produce naphthalene ester, solid blue color development. Determination of 450 nm light absorption increase rate could calculate CarE activity.
Self Provided:
Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom plate, Low Temperature Centrifuge, Water-Bath, Adjustable Pipette, Distilled Water, Anhydrous Ethanol.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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