Chitinase Activity Assay Kit
Cat.No：BC0825 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > Chitinase Activity Assay Kit
Product Information
| Name | Chitinase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Chitinases are found in the shells of crustaceans such as shrimps, crabs and insects, and in the organs of mollusks (such as squid cartilage), as well as in the cell walls of fungi. Chitinase (EC 3.2.1.14) can catalyze the hydrolysis of chitin, which has the function of resisting fungal infection and become the research hotspot of antifungal diseases.
Measurement Principle：
Chitinase hydrolyzes chitin to produce N-acetylglucosamine, and the intermediate compound produced by co-heating of N-acetylglucosamine with alkali can further react with p-dimethylaminobenzaldehyde to produce a chromogenic substance, which has the characteristic absorption peaks at 585nm, and the rate of increase in absorbance reflects the activity of chitinase.
Self Provided：
Scales, water bath/constant temperature incubator, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, distilled water, ultrasonic crusher.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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