Hexokinase(HK) Activity Assay Kit
Cat.No：BC0745 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Hexokinase(HK) Activity Assay Kit
Product Information
| Name | Hexokinase(HK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Hexokinase (HK, EC 2.7.1.1) is widely distributed in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose into glucose 6-phosphate, which is the intersection of glycolysis and pentose phosphate pathways.
Measurement Principle：
HK catalyzes the synthesis of glucose to 6-phosphate glucose, and 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of 6-phosphate glucose to NADPH, which has a characteristic absorption peak at 340 nm.
Self Provided：
Spectrophotometer/microplate reader, water-bath, table centrifuge, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer and ice.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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