Pyruvate Carboxylase(PC) Activity Assay Kit
Cat.No：BC0735 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Gluconeogenic Series > Pyruvate Carboxylase(PC) Activity Assay Kit
Product Information
| Name | Pyruvate Carboxylase(PC) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Pyruvate carboxylase (PC, EC 6.4.1.1) is widely present in mitochondria of animals, molds and yeast, but is not found in plants and most bacteria. PC is the main postreaction for oxaloacetate, and is the first rate-limiting enzyme in the gluconeogenesis process.
Measurement Principle：
PC irreversibly catalyzes pyruvate, ATP, CO2 and water to oxaloacetate, ADP and Pi, malic dehydrogenase further catalyzes the formation of malic acid and NAD+ from acetoacetic acid and NADH. The enzyme activity of PC can be reflected by detecting the oxidation rate of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Pyruvate Carboxylase Activates the RIG-I-like Receptor-Mediated Antiviral Immune Response by Targeting the MAVS signalosome
Click to check >>Author：Zhongying Cao, Yaqin Zhou, Shengli Zhu, Jian Feng,
IF：4.2590
Publish_to：Scientific Reports 6, Article number: 22002 (2016)
PMID：26906558
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