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α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit

BC0715Manual

Cat.No：BC0715 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is one of the key enzymes in the regulation of tricarboxylic acid cycle and widely exists in mitochondria of animal, plant, microorganisms and cultured cells, which catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl coenzyme A.

Measurement Principle：

α-KGDH catalyzes α-ketoglutarate, NAD+ and coenzyme A to form succinyl coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. The activity of α-KGDH is expressed by the formation rate of NADH.

Self Provided：

Ultraviolet spectrophotometer/microplate reader, water-bath/constant temperature incubator, tabletop centrifuge, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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