Lacate Dehydrogenase(LDH) Activity Assay Kit
Cat.No：BC0685 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Lacate Dehydrogenase(LDH) Activity Assay Kit
Product Information
| Name | Lacate Dehydrogenase(LDH) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
L-Lactate dehydrogenase (L-LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). L-LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.
Measurement Principle：
NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of L-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.
Self Provided：
Spectrophotometer/Microplate reader, thermostat water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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