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CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)

BC5205Manual

Cat.No：BC5205 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

Coenzyme II NADP(H) is widely present in animals, plants, microorganisms and cultured cells. The determination of NADP+ and NADPH content can calculate the content of NADP (NADPH + NADP+) and the ratio of NADPH/NADP+, and its changes are related to the pentose phosphate pathway and biosynthesis and Antioxidative responses are closely related. The NADPH/NADP+ ratio is not only one of the main markers of cellular redox state, but also plays an important regulatory role in the PPP pathway, biosynthesis and antioxidant metabolism.

Measurement Principle：

The NADP+ and NADPH in the samples were extracted with acidic and basic extraction solutions, respectively. Under the action of 1-mPMS, WST-1 can react with NADPH to produce water-soluble formazan, which has a characteristic absorption peak at 450nm, while NADP+ can be reduced to NADPH by 6-phosphate glucose dehydrogenase, which is further detected by WST-1.

Self Provided：

Spectrophotometer/microplate reader, centrifuge, water bath, mortar/homogenizer, sonicator, adjustable pipette, micro glass cuvettes/96 well plates, ice, and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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