α-amylase (α-AL) Activity Assay Kit
Cat.No：BC0615 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Starch Series > α-amylase (α-AL) Activity Assay Kit
Product Information
| Name | α-amylase (α-AL) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Amylase including α-amylase and β-amylase. α-amylase (α-AL, EC 3.2.1.1) randomly catalyze the hydrolysis of α-1,4-glycosidic bonds in starch to produce reducing sugars such as glucose, maltose, maltotriose, dextrin, etc. At the same time, the viscosity of starch is reduced, so it is also called liquefied enzyme.
Measurement Principle：
Starch hydrolase catalyzes the hydrolysis of starch to produce reducing sugar. 3,5-dinitrosalicylic acid is reduced to brown red substance by the reducing sugar, and the brown red substance has an absorption peak at 540 nm. The activity of amylase is calculated by measuring the increasing rate of absorbance at 540 nm. α-AL is thermostable, but β-AL could be passivated at 70℃ for 15 minutes. Therefore, only α-AL could catalyze starch hydrolysis when the crude enzyme solution is passivated at 70℃ for 15 minutes.
Self Provided：
Spectrophotometer/microplate reader, thermostat water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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调整待测样本量的具体比例 2022-07-23 14:47:58
Administrator您好，测定的吸光值大于1.5时，可以对样本进行适当稀释后测定。若吸光值过小，可以浓缩淀粉酶稀释液或者淀粉酶原液。稀释样本可以按倍比稀释的方法做不同浓度梯度的，如果能落在标曲范围内就不需要进一步再稀释了