Phosphofructokinase(PFK) Activity Assay Kit
Cat.No：BC0535 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Phosphofructokinase(PFK) Activity Assay Kit
Product Information
| Name | Phosphofructokinase(PFK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
PFK (EC 2.7.1.11) is one of the key regulatory enzymes in the process of glycolysis, which widely found in animals, plants, microorganisms and cultured cells. It is responsible for converting fructose-6-phosphate and ATP into fructose-diphosphate and ADP.
Measurement Principle：
PFK catalyzes the formation of fructose-1,6-diphosphate and ADP to from fructose-6-phosphate and ATP. Pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to generate NAD+. The degradation rate of NADH which measured at 340 nm is used to reflect the activity of PFK.
Self Provided：
Spectrophotometer/microplate reader, table centrifuge, water-bath, micro quartz cuvette/96 well flat-bottom UV plate, adjustable pipette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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