Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit
Cat.No：BC0445 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme I Series > Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit
Product Information
| Name | Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Ribulose 1,5-bisphosphate carboxylase/oxygenase(Rubisco) is a key enzyme in plant photosynthesis, which controls the carbon dioxide fixation, and restricts the shunt of carbon into the Calvin cycle and photorespiration cycle. The activity of rubisco has direct reflect on the photosynthetic rate.
Measurement Principle：
Rubisco catalyzes combination of one molecule of ribulose-1,5-diphosphate (RuBP) binds and one molecule of carbon dioxide to produce two molecules of 3-phosphoglycerate (PGA). PGA produces glyceraldehyde-3-phosphate by the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, which is accompanied by NADH oxidation to form NAD+. NADH has a characteristic absorption peak at 340 nm, while NAD+ does not. In this kit, the activity of rubisco is determined by the decrease rate of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate Reader, desk centrifuge, adjustable pipette, water bath, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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