ADPG Pyrophosphorylase(AGP) Activity Assay Kit
Cat.No：BC0435 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Starch Series > ADPG Pyrophosphorylase(AGP) Activity Assay Kit
Product Information
| Name | ADPG Pyrophosphorylase(AGP) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
ADPG Pyrophosphorylase (AGP) exists mainly in plants, is the main rate-limiting step in plant starch biosynthesis, which catalyzes the reaction of glucose-1-phosphate (G1P) with ATP to produce direct precursor adenosine diphosphate glucose (ADPG) for starch synthesis.
Measurement Principle：
AGP catalyzes the reverse reaction to produce G1P, the added phosphate hexose mutase and 6-phosphate glucose dehydrogenase catalyze the formation of 6-phosphate gluconate and NADPH. In this kit, the activity of AGP is determined by the increase rate of NADPH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/ microplate reader, water bath/incubator, benchtop centrifuge, adjustable pipette, 1mL quartz cuvette, mortar/homogenizer, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
The SnRK2.3-AREB1-TST1/2 cascade activated by cytosolic glucose regulates sugar accumulation across tonoplasts in apple and tomato
Click to check >>Author：Zhu Lingcheng, Li Yanzhen, Wang Chengcheng, Wang Zhiqi, Cao Wenjing, Su Jing, Peng Yunjing, Li Baiyun, Ma Baiquan, Ma Fengwang, Ruan Yong-Ling, Li Mingjun
IF：18.0000
Publish_to：Nature Plants
PMID：37291399
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