Acetyl CoA carboxylase(ACC) Activity Assay Kit
Cat.No：BC0415 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Fatty Acid Metabolism Series > Acetyl CoA carboxylase(ACC) Activity Assay Kit
Product Information
| Name | Acetyl CoA carboxylase(ACC) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl CoA to malonyl CoA in vivo and is a key enzyme in the synthesis of fatty acids and many secondary metabolites. The activity of ACC determines the synthesis speed and oil content of fatty acids to a certain extent.
Measurement Principle：
ACC can catalyze acetyl coenzyme A, NaHCO3 and ATP to generate malonyl CoA, ADP and inorganic phosphorus. Molybdenum blue and phosphate generate a substance with a characteristic absorption peak at 660nm. ACC activity is determined by measuring the increase of inorganic phosphorus by ammonium molybdate phosphorus determination method.
Self Provided：
Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice, Low Temperature Centrifuge, Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/96 Well Flat-bottom Plate (UV plate), Water Bath; Adjustable Pipette and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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