Pyruvate Dehydrogenase(PDH) Activity Assay Kit
Cat.No：BC0385 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Tricarboxylic Acid Cycle Series > Pyruvate Dehydrogenase(PDH) Activity Assay Kit
Product Information
| Name | Pyruvate Dehydrogenase(PDH) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
PDH widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex (PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl-TPP, links glycolysis to the three carboxylic acid cycle.
Measurement Principle：
PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-DCPIP), which makes the absorption of 605 nm decrease.
Self Provided：
Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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