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Application： ELISA
Reactivity：Mouse
Sensitivity：78.13 pg/ml
Storage：2-8℃，6 months
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| Name | Mouse Wnt-3a ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Wnt-3a |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 156.25-10000 pg/ml |
| Sensitivity | 78.13 pg/ml |
| Specificity | Do not react with Mouse FRP-1，FRP-2，FRP-4，LRP-6，Nurr-1，Wnt-4，Wnt-5b etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P27467 |
| Gene ID | 22416 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠Wnt-3a单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠Wnt-3a会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠Wnt-3a抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠Wnt-3a发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠Wnt-3a，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠Wnt-3a浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠Wnt-3a的浓度。 |
| Background | Wnt-3a is a secreted protein that is necessary for proper embryonic development of the hippocampus，anterior-posterior patterning，somite development，and tailbud formation. It also promotes self-renewal of hematopoietic stem cells，neural stem cells，and embryonic stem cells. The post-translational glycosylation and acylation of Wnts are essential for their efficient secretion and biological activity，respectively. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels. As intracellular beta-catenin levels rise，beta-catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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