Fura-2 AM
Cat.No：IF1510 Solarbio
CAS：108964-32-5
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥95%
Appearance：Solid
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PI3K/Akt/mTOR
Endocrinology & Hormones
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Angiogenesis
HIF
Immunology & Inflammation
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Apoptosis
Metabolic Enzyme & Protease
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ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
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Ubiquitin
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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Product Information
| CAS | 108964-32-5 |
| Name | Fura-2 AM |
| Molecular Formula | C44H47N3O24 |
| Molecular Weight | 1001.85 |
| Solubility | Soluble in DMSO |
| Purity | ≥95% |
| Appearance | Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00036976 |
| SMILES | O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O |
| Background | It is a high-affinity calcium ion fluorescent probe that can permeate cells. |
| Unit | Bottle |
| Specification | 50ug 1mg |
FURA-2AM is a high affinity calcium ion fluorescent probe that is permeable to cells. FURA-2AM is an ester form of FURA-2, a highly polar acidic compound that cannot enter living cells, and the addition of acetoxymethyl (AM) to its negative group significantly increases cell permeability. After penetrating the cell membrane into living cells, FURA-2AM is de-esterified by intracellular esterase into FURA-2 free acid, which binds specifically to cytoplasmic free Ca2+ (binding ratio 1:1). When FURA-2 binds to free Ca2+ in the cytoplasm, it exhibits an absorption shift, with the excitation wavelength peak changing from 380 nm to 340 nm, while the emission peak near 510 nm remains unchanged. There was a quantitative relationship between fluorescence intensity and the concentration of bound Ca2+. Fura-2 is generally excited at 340 nm (calcium ion -Fura 2) and 380nm (Fura 2) wavelengths, and the intracellular calcium ion concentration is calculated by using the ratio of fluorescence intensity corresponding to the two excitations. Ratio measurements minimize the effects of photobleaching in the mixture, leakage of fluorescent probes, uneven loading of probes and differences in cell thickness, providing more reliable and reproducible results.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Click to check >>Author：Haishan Qi, Yihang Gao, Lin Zhang, Zhongxin Cui, Xiaojie Sui, Jianfan Ma, Jing Yang, Zhiquan Shu, Lei Zhang
IF：12.8000
Publish_to：Engineering
PMID：
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