Prussian Blue Iron Stain Kit(Ferric Iron，Enhance With DAB)
Cat.No：G1428 Solarbio
Storage：Store at 2-8℃，avoid light，1 year
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> Dye Reagents > Intracellular Component Stain > Prussian Blue Iron Stain Kit(Ferric Iron，Enhance With DAB)
Product Information
| Name | Prussian Blue Iron Stain Kit(Ferric Iron，Enhance With DAB) |
| Storage | Store at 2-8℃，avoid light，1 year |
| Unit | Kit |
| Specification | 4*10ml 4*20ml |
Product Introduction:
The Prussian Blue Reaction is a method of staining non heme iron (containing heme, iron transporters, iron deposition, etc.) in cells or tissues containing granular iron. It plays an important role in the validation of diseases such as ischemic anemia, hemolytic anemia, hemochromatosis, and abnormal hemoglobin metabolism, and can also be used in conjunction with other enhancers for the detection of iron death models.
The Prussian blue reaction is limited by the reaction principle and imaging equipment accuracy, and can only be used for observing iron overload or iron deposition staining. The staining effect is poor for samples with low iron content or non iron overload type iron death models. The Prussian Blue Iron Stain Kit (Ferric Iron,Enhance With DAB) introduces an enhancer on the basis of conventional Prussian blue staining, and uses the principle of cascade amplification to amplify and display the positive signal. It is suitable for trivalent iron staining of tissues with less iron content or less iron deposition.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
小鼠正常脑组织石蜡切片左半边为增强型普鲁士蓝染色结果，铁阳性位点呈黄褐色，背景呈淡黄色或组织本色。右半边为苏木素复染结果，阳性位点着黄褐色，细胞核着蓝色，背景着淡蓝色或组织本色。
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是否可用于铁死亡过程中铁沉积染色 2023-03-12 11:08:27
Administrator理论上是可以的，如果铁沉积比较少，可能染色就比较困难。
细胞染色需要固定吗 2022-09-05 21:16:57
Administrator4%多聚甲醛固定，避免使用酸性固定剂固定
怎么定量
2022-07-28 21:18:49
Administrator您好，染色后做定性观察的，定量分析您再查一下文献中有没有分析方法吧