β-Hydroxybutyric acid (β-HB) Content Assay Kit
Cat.No：BC5080 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme I Series > β-Hydroxybutyric acid (β-HB) Content Assay Kit
Product Information
| Name | β-Hydroxybutyric acid (β-HB) Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
β-Hydroxybutyric acid (β-HB), in patients with severe acidosis, NADH production increases due to acidosis, which in turn promotes the ratio of β-hydroxybutyric acid to acetoacetic acid to increase from the normal 2:1 to 16: 1. β-hydroxybutyric acid is of great significance in the diagnosis and treatment of diabetic ketoacidosis. It is also of great significance for the early diagnosis of diabetes. The kit is suitable for serum, plasma, urine and other liquid samples.
Measurement Principle：
At pH8.8 and 37℃, β-hydroxybutyrate reacts under the catalysis of β-hydroxybutyrate dehydrogenase, and NAD+ is oxidized to NADH. In the presence of 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan with a characteristic absorption peak at 450nm. The content of β-HB can be calculated by detecting the wavelength change at 450nm.
Self Provided：
Spectrophotometer, desk centrifuge, pipette, 1mL glass cuvette, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Multi-omics analysis reveals that the metabolite profile of raw milk is associated with dairy cows’ health status
Click to check >>Author：Yuchao Zhao, Huiying Zhao, Liuxue Li, Jian Tan, Ying Wang, Ming Liu, Linshu Jiang
IF：8.8000
Publish_to：FOOD CHEMISTRY
PMID：37421666
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